Proteomics Specialist

Scientific Topics

Website John Hopkins University

General Summary/Purpose

We are presently seeking a very-disciplined and highly self-motivated individual to assist in the group as a Proteomics Specialist to join the Mass Spectrometry and Proteomics Facility at Johns Hopkins School of Medicine, Baltimore, MD. The individual should have a sophisticated understanding of techniques and a solid foundation in the underlying science, gained from prior research experience. The successful candidate will develop and implement state-of-the-art mass spectrometry-based methods to identify, characterize and quantify proteins from complex protein mixtures such as cell lysates and human plasma samples submitted by Johns Hopkins Investigators. In addition, the candidate will operate and maintain a high-resolution tandem mass spectrometers such as Thermo Scientific Orbitrap Lumos and Q-Exactive and the requisite nano-scale liquid chromatography and Capillary Electrophoresis systems.

Specific Duties & Responsibilities

Operate and maintain highly technical mass spectrometers and two-dimensional chromatographic or electrophoresis equipment.  Identify, advise or assist investigators with developing strategies for sample preparation for the use of gel electrophoresis, liquid chromatography, capillary electrophoresis and mass spectrometry to identify, quantify and characterize proteins and their modifications. Expand or develop new skills, as needed, based on information from literature, in collaboration with other laboratories or vendors, or by interacting with colleagues at meetings or professional presentations.  Teach staff, students or fellows advanced techniques in protein analysis by mass spectrometry used in the center through informal discussions or formal workshops offered by the facility. The position also implies daily supervision of all aspects of the center functioning. The proteomics specialist is responsible for maintaining high-end mass spectrometers, developing and following SOPs, developing and maintaining collaborative connections with the investigators. The successful candidate will be involved in biomedical projects. Independent research in technological developments in proteomics will be strongly encouraged.

Instrumentation handled:               

Mass Spectrometers (Orbitraps Lumos, Lumos ETD, Q-Exactive Plus, Voyager DE-STR MALDI-TOF)

Liquid chromatography (nanoflow, capillary flow, affinity, reverse phase, cationic exchange)

Capillary Electrophoresis

Minimum Qualifications

Master’s degree in biology, chemistry or related experience is required.

Two (2) years’ experience with protein/peptide separation and analysis by liquid chromatography – mass spectrometry is required.

* JHU Equivalency Formula18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for the required education on the same basis. 

Preferred Qualifications

Master’s degree in biology, chemistry or related experience is required.

Four (4)+ years’ experience with protein/peptide separation and analysis by liquid chromatography – mass spectrometry is required.

Special Knowledge, Skills, and Abilities

Expertise in quantitative proteomics using label free (PRM, DIA) or labeling techniques (iTRAQ, TMT, SILAC or NeuCode)

Individual must have good communication skills, be able to work independently, under minimal supervision with strong decision making skills and good judgment.

Classified Title: Proteomics Specialist
Working Title: Proteomics Specialist
Role/Level/Range: ACRP/04/ME
Starting Salary Range: $60,945-$83,865/ commensurate with experience
Employee group: Full Time
Schedule: Monday – Friday, 8:30 a.m – 5:00p.m/ 37.5 Hrs/Wk
Exempt Status: Exempt
Location: 04-MD:School of Medicine Campus
Department name: 60002528-BiolChem Fac/Staff
Personnel area: School of Medicine

The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at For TTY users, call via Maryland Relay or dial 711.

The following additional provisions may apply depending on which campus you will work.  Your recruiter will advise accordingly.

During the Influenza (“the flu”) season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

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School of Medicine – East Baltimore Campus

Personnel Area
School of Medicine


Johns Hopkins University is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria. The university promotes affirmative action for minorities, women, disabled persons, and veterans.

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