bioRxiv
Structure−function properties in disordered condensates
Jill Bouchard
Contributing editor, Condensates.com
It's interesting to see how the structures outside and inside condensates matter for function.
Must Reads
Nature
Composition-dependent Thermodynamics of Intracellular Phase Separation
Nature methods
Pooled CRISPR Screens With Imaging on Microraft Arrays Reveals Stress Granule-Regulatory Factors
Jill Bouchard
Contributing editor, Condensates.com
CRISPR + machine learning + stress granules = really cool stuff!
Cell
Unblending of Transcriptional Condensates in Human Repeat Expansion Disease
Jill Bouchard
Contributing editor, Condensates.com
It's not surprising that repeat expansions alter the phase separation and subsequent material properties of condensates. The discussion on how poly-alanine (and other IDR) tracts contribute to disease will be super useful going forward.
Cell
Condensation of Ded1p Promotes a Translational Switch From Housekeeping to Stress Protein Production
Cell
Networking and Dynamic Switches in Biological Condensates
Jill Bouchard
Contributing editor, Condensates.com
Here's the Preview for the trifecta of papers in Cell this week about stress granules and LLPS. See the papers from the Brangwynne/Ivanov labs, Alberti/Pappu/Hyman labs, and the Taylor/Mittag labs.
Cell
Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization
Jill Bouchard
Contributing editor, Condensates.com
This is fascinating work outlining the interactions that determine the composition and miscibility of stress granules and P bodies!
Cell
G3BP1 Is a Tunable Switch That Triggers Phase Separation to Assemble Stress Granules
Jill Bouchard
Contributing editor, Condensates.com
Here's a great example of how IDRs can fine-tune the formation of RNP condensates.
Cell
RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation
Jill Bouchard
Contributing editor, Condensates.com
I like how this work was able to break down RNP condensation to a single protein's conformational switch.
Nature
LRP1 Is a Master Regulator of Tau Uptake and Spread
Jill Bouchard
Contributing editor, Condensates.com
Here's some stellar work showing that LRP1 interacts w/ lysine residues in Tau, allowing Tau to spread to other neurons.
Nature physics
Elastic Ripening and Inhibition of Liquid-Liquid Phase Separation
bioRxiv
TDP-43 and HSP70 phase separate into anisotropic, intranuclear liquid spherical annuli
Nature
Phase Separation Directs Ubiquitination of Gene-Body Nucleosomes
Jill Bouchard
Contributing editor, Condensates.com
Cool! Layered condensates act like reaction chambers around nucleosomes!
bioRxiv
A quantitative inventory of yeast P body proteins reveals principles of composition and specificity
Jill Bouchard
Contributing editor, Condensates.com
This could be a very enlightening method to study for any condensate.
Nature cell biology
Phase Separation of TAZ Compartmentalizes the Transcription Machinery to Promote Gene Expression
Cell
Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting
Science (New York, NY)
Liquid-liquid phase separation drives skin barrier formation
Proceedings of the National Academy of Sciences of the United States of America
Composition-based Prediction and Rational Manipulation of Prion-Like Domain Recruitment to Stress Granules
Other resources
Biomolecular condensates for beginners—and aliens
Annual review of physical chemistry
Single-Molecule FRET of Intrinsically Disordered Proteins
Jill Bouchard
Contributing editor, Condensates.com
I bet the methods discussed will be super-useful for studying condensates.