Current opinion in cell biology
Most Recent
Journal of molecular cell biology
Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assemblies
Journal of molecular biology
Biochemical timekeeping via reentrant phase transitions
PloS one
Assessing the low complexity of protein sequences via the low complexity triangle
Jill Bouchard
Contributing editor, Condensates.com
Do you need a new angle to look at an IDP or LCD to understand why it behaves a certain way in a condensate? Maybe this method will help.
BioRxiv
Inference of emergent spatio-temporal processes from single-cell sequencing reveals feedback between de novo DNA methylation and chromatin condensates
Science
In situ genome sequencing resolves DNA sequence and structure in intact biological samples
Jill Bouchard
Contributing editor, Condensates.com
I have a feeling this will be a useful new method for condensates researchers.
bioRxiv
Multivalent polymers can control phase boundary, dynamics, and organization of liquid-liquid phase separation
The journal of physical chemistry B
Conformational Preferences of an Intrinsically Disordered Protein Domain: A Case Study for Modern Force Fields
Jill Bouchard
Contributing editor, Condensates.com
If you're on the market to find the best way to simulate a phase separating IDP, this should be a good resource.
Analytical Chemistry
Monitoring the Dynamics of Proteome Aggregation in Live Cells Using a Solubilized and Noncovalent Analogue of Fluorescent Protein Chromophores
Jill Bouchard
Contributing editor, Condensates.com
Cool! This new sensor can detect liquid-to-solid transitions with 2 different colors.
Journal of the American Chemical Society
Effects of Glutamate Arginylation on α-Synuclein: Studying an Unusual Post-Translational Modification through Semisynthesis
Jill Bouchard
Contributing editor, Condensates.com
I could see this being an effective strategy to interrogate the effects of unusual PTMs on proteins involved in LLPS too.
The journal of physical chemistry B
Liquid-Liquid Phase Separation of Peptide/Oligonucleotide Complexes in Crowded Macromolecular Media
BioRxiv
Hsp70 chaperons TDP-43 in dynamic, liquid-like phase and prevents it from amyloid aggregation
bioRxiv
Oxidized RNA Bodies compartmentalize translation quality control in Saccharomyces cerevisiae
Jill Bouchard
Contributing editor, Condensates.com
Here's an update to the paper about the new condensate (ORBs) that communicate with stress granules and P bodies.
BioRxiv
Amyloid fibril-based hydrogels for high-throughput tumor spheroid modeling
bioRxiv
A comprehensive motifs-based interactome of the C/EBPα transcription factor
Jill Bouchard
Contributing editor, Condensates.com
The authors point out that this method may be a useful for identifying important interactions and PTM regulations of IDPs involved in condensates.
bioRxiv
PARprolink: a photoaffinity probe for identifying poly(ADP-ribose)-binding proteins
Jill Bouchard
Contributing editor, Condensates.com
Not only did these authors develop a new method to identify PAR-binding proteins, they also found them to be enriched in condensates. Read further to find out which ones.
bioRxiv
Solubility product constant directs the formation of biomolecular condensates
ACS nano
Tunable Electromechanical Nanopore Trap Reveals Populations of Peripheral Membrane Protein Binding Conformations
Jill Bouchard
Contributing editor, Condensates.com
I wonder if this this nanopore could be used to investigate proteins involved in membrane-associated condensates too?
bioRxiv
RNA polymerase II condensate formation and association with Cajal and histone locus bodies in living human cells
Microorganisms
Escherichia coli HS and Enterotoxigenic Escherichia coli Hinder Stress Granule Assembly
Jill Bouchard
Contributing editor, Condensates.com
It looks like both good and bad kinds of strains of E. coli have an interesting relationship with stress granules. Check out the details to learn more.