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NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency

Authors Kyoung-Jae Choi  , My Diem Quan  , Chuangye Qi  , Joo-Hyung Lee , Phoebe S Tsoi , Mahla Zahabiyon , Aleksandar Bajic , Liya Hu , B V Venkataram Prasad , Shih-Chu Jeff Liao , Wenbo Li  , Allan Chris M Ferreon , Josephine C Ferreon 
Details Kyoung-Jae Choi et al; Nat Cell Biol; 2022;
DOI 10.1038/s41556-022-00896-x
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Sidharth Sirdeshmukh Recent comment authors
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Sidharth Sirdeshmukh
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Sidharth Sirdeshmukh
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NANOG is a transcription factor that can reestablish ground-state pluripotency in stem cells. The authors of this paper sought to investigate the LLPS dynamics that resulted from amino acid-level differences in NANOG c-termini, that may drive this effect. Changing all tryptophans to alanines (WT to W8A) resulted in smaller monomeric assemblies of NANOG, which was not favorable for droplet formation. Additionally, FRAP experiments confirmed that these larger WT assemblies recover from photobleaching more slowly than W8A. CHIP-seq experiments show that these amino acid changes influence the regions of chromatin DNA recognized by WT and W8A, which connects these findings to… Read more »