Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching
Authors | Taylor NO, Wei MT, Stone HA, Brangwynne CP |
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Details | Biophys J. 2019 Oct 1;117(7):1285-1300. doi: 10.1016/j.bpj.2019.08.030. Epub 2019 Aug 30 |
DOI | 10.1016/j.bpj.2019.08.030 |
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Source | View on PubMed |
FRAP is a used commonly to show that condensates have liquid properties, but what does it really tell us? This is worth a read to make sure you are analyzing your data correctly and drawing the right conclusions.