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Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching

Authors Taylor NO, Wei MT, Stone HA, Brangwynne CP
Details Biophys J. 2019 Oct 1;117(7):1285-1300. doi: 10.1016/j.bpj.2019.08.030. Epub 2019 Aug 30
DOI 10.1016/j.bpj.2019.08.030
Source View on PubMed

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Jill Bouchard
Jill Bouchard
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FRAP is a used commonly to show that condensates have liquid properties, but what does it really tell us? This is worth a read to make sure you are analyzing your data correctly and drawing the right conclusions.