Rotavirus Replication Factories Are Complex Ribonucleoprotein Condensates

Authors Florian Geiger, Guido Papa, William E Arter, Julia Acker, Kadi Liis Saar, Nadia Erkamp, Runzhang Qi, Jack P.K. Bravo, Sebastian Strauss, Georg Krainer, Oscar R. Burrone, Ralf Jungmann, Tuomas Knowles, Hanna Engelke, Alexander Borodavka
Details bioRxiv. December 20, 2020
DOI 10.1101/2020.12.18.423429
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Alex Borodavka
Alex Borodavka
Dr, University of Cambridge

Despite extensive biochemical characterization of viral replication factories in rotaviruses, the origins of these electron-dense membraneless inclusions in virus-infected cells have remained a mystery for over 40 years. In this manuscript the authors provide the first evidence that rotavirus replication factories represent complex biomolecular condensates that undergo phase transitions during the course of infection. The authors reconstitute protein condensates in vitro, and show that these condensates are equally sensitive to 1,6-hexanediol in vitro, and in vivo, in virus-infected cells. Remarkably, the early infection factories appear to be sensitive to a number of small aliphatic diols, including propylene glycol, a generally… Read more »