bioRxiv
Science
British journal of pharmacology
Targeting TDP-43 Proteinopathy With Drugs and Drug-Like Small Molecules
Jill Bouchard
Contributing editor, Condensates.com
This review covers how some drugs affect TDP-43 mislocalization.
Scientific reports
Dengue Virus Capsid Protein Dynamics Reveals Spatially Heterogeneous Motion in Live-Infected-Cells
Jill Bouchard
Contributing editor, Condensates.com
It looks like dengue viral capsid phase separates and joins the nucleolus as part of it's invasion strategy... another way (of the many) that viruses use our condensates against us.
Current opinion in cell biology
Karyopherins and Condensates
Trends in biochemical sciences
Mechanisms and Regulation of RNA Condensation in RNP Granule Formation
Experimental cell research
Cellular Stress Orchestrates the Localization of hnRNP H to Stress Granules
Jill Bouchard
Contributing editor, Condensates.com
Many of the hnRNP-family proteins have been shown to localize to stress granules. It looks like the hnRNP H is no different.
The Journal of Biological Chemistry
Phase-separated Condensate-Aided Enrichment of Biomolecular Interactions for High-Throughput Drug Screening in Test Tubes
Molecular Cell
Sequence-Independent Self-Assembly of Germ Granule mRNAs Into Homotypic Clusters
Journal of molecular cell biology
The Protective Role of m1A During Stress-Induced Granulation
Jill Bouchard
Contributing editor, Condensates.com
Here's the second article this week showing a role of mRNA methylation in condensate formation... only this time it's m1A (where the N1 A gets post-transcriptionally modified).
The FEBS journal
The More the Merrier: Effects of Macromolecular Crowding on the Structure and Dynamics of Biological Membranes
Jill Bouchard
Contributing editor, Condensates.com
This review covers 2 types of condensates lipid-droplets and protein-phase separation at and near membranes. Check it out if you'd like to learn about the latest developments.
Protein & cell
Live Cell Imaging and Proteomic Profiling of Endogenous NEAT1 lncRNA by CRISPR/Cas9-mediated Knock-In
Jill Bouchard
Contributing editor, Condensates.com
Cool. Here's a new method to track endogenous lncRNA in cells and identify proteins it may interact with, whereby they identified new proteins in paraspeckles.
bioRxiv
A selective autophagy pathway for phase separated endocytic protein deposits
Nature chemical biology
M 6 A-binding YTHDF Proteins Promote Stress Granule Formation
Jill Bouchard
Contributing editor, Condensates.com
ICYMI m6A is methylated mRNA (on the N6 of the adenine), so post-transcriptional modifications play a role in condensate formation just as much as post-translational ones.
Small (Weinheim an der Bergstrasse, Germany)
Invasion and Defense Interactions Between Enzyme-Active Liquid Coacervate Protocells and Living Cells
Chemistry (Weinheim an der Bergstrasse, Germany)
Alteration of the Conformational Dynamics of a DNA Hairpin by α-Synuclein in the Presence of Aqueous Two-Phase Systems
Jill Bouchard
Contributing editor, Condensates.com
This study applies pressure to a phase-separated neuro-protein interacting with DNA to understand how the DNA is altered long-term.
PLoS pathogens
RNF8 Dysregulation and Down-regulation During HTLV-1 Infection Promote Genomic Instability in Adult T-Cell Leukemia
Jill Bouchard
Contributing editor, Condensates.com
Interesting stuff: These authors show that upon infection of a leukemia-linked retrovirus, important DNA repair enzymes get sequestered away in Tax speckle structures, which leads to further genomic instability in leukemia cells.
BioRxiv
Multivalent proteins rapidly and reversibly phase-separate upon osmotic cell volume change
BioRxiv
The cryoEM structure of the fibril-forming low-complexity domain of hnRNPA2 reveals distinct differences from pathogenic amyloid and shows how mutation converts it to the pathogenic form
Jill Bouchard
Contributing editor, Condensates.com
Interesting... these authors show reversible hydrogels contain amyloid-like fibrils.
BioRxiv
Membrane bending by protein phase separation
Cells