Nuclear speckles

Developmental cell

N 6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles

• Cancer
• Imaging
• Physics of Condensates

Jill Bouchard

Contributing editor, Condensates.com

It looks like this lncRNA could be an important scaffold for nuclear speckles. Check out the paper for all the details.

Biochimica et biophysica acta Molecular and cell biology of lipids

Nanoscale mapping of nuclear phosphatidylinositol phosphate landscape by dual-color dSTORM

• Methods
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

Here's a method (and a good reminder) to think about lipids too when studying condensates.

bioRxiv

SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNP complexes

• Methods
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

Here's a new protein associated with Cajal bodies and nuclear speckles.

Current opinion in cell biology

Phosphorylation-dependent regulation of messenger RNA transcription, processing and translation within biomolecular condensates

• Metabolism
• Phase Separation
• Transcription

Genome biology

SPIN reveals genome-wide landscape of nuclear compartmentalization

• Methods
• Transcription

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Regulation of the nuclear speckle localization and function of Rac1

• Cancer
• Transcription

PLoS genetics

Dynamic regulation of CTCF stability and sub-nuclear localization in response to stress

• Cancer
• Methods

Jill Bouchard

Contributing editor, Condensates.com

Interesting--it looks like the authors were able to change the compositions of nuclear speckles with stress.

A Newfound Source of Cellular Order in the Chemistry of Life

• Neurodegeneration
• Phase Separation
• Physics of Condensates
• Transcription

Cells

Limited Proteolysis-Coupled Mass Spectrometry Identifies Phosphatidylinositol 4,5-Bisphosphate Effectors in Human Nuclear Proteome

• Cancer
• Methods
• Phase Separation
• Physics of Condensates

Jill Bouchard

Contributing editor, Condensates.com

Do lipids make it into condensates? This method seeks to uncover the complicated relationship between lipids and many proteins that localize to condensates.

Genome research

TSA-seq reveals a largely conserved genome organization relative to nuclear speckles with small position changes tightly correlated with gene expression changes

• Transcription

Jill Bouchard

Contributing editor, Condensates.com

Who knew nuclear speckles had such a large role in regulating gene expression?!

Nucleic acids research

Splicing at the phase-separated nuclear speckle interface: a model

• Phase Separation
• Physics of Condensates

Current opinion in genetics & development

Nuclear mechanisms of gene expression control: pre-mRNA splicing as a life or death decision

• Physics of Condensates
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

How do nuclear speckles play a role in splicing? Read this to find out.

The EMBO journal

Clustering and reverse transcription of HIV-1 genomes in nuclear niches of macrophages

• Imaging
• Infectious Disease
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

It looks like the condensates in infected macrophages get all messed up. Check out this paper to find out more.

eLife

Viral-induced alternative splicing of host genes promotes influenza replication

• Infectious Disease
• Physics of Condensates
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

It looks like nuclear speckles may be a key location for the splicing agents that help this virus with replication.

bioRxiv

SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNPs from Cajal bodies to nuclear speckles for spliceosome assembly

• Methods
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

This paper implicates nuclear speckles and cajal bodies in the cellular dysfunctions that may lead to deaf-blindness.

Briefings in bioinformatics

MloDisDB: a manually curated database of the relations between membraneless organelles and diseases

• Cancer
• Neurodegeneration
• Phase Separation

Jill Bouchard

Contributing editor, Condensates.com

Do you want to know what disease(s) your favorite protein or condensate is associated with? You can use this online platform to find out. I’ve saved it in our library of Resources–check it out here: https://condensates.com/resources/mlodisdb/

Scientific reports

A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice

Jill Bouchard

Contributing editor, Condensates.com

Interesting--a mutation associated with heart disease causes a protein not to localize to nuclear speckles, but instead it accumulates in cytoplasmic granules of some sort. I wonder it this mutation alters its phase separation propensity or just alters it's miscibility?

eLife

SON and SRRM2 are essential for nuclear speckle formation

• Methods

Jill Bouchard

Contributing editor, Condensates.com

Have you used SC35 to mark nuclear speckles? Then you're probably going to want to read why it actually localizes to them.

Nucleic acids research

TPR is required for the efficient nuclear export of mRNAs and lncRNAs from short and intron-poor genes

• Transcription

Jill Bouchard

Contributing editor, Condensates.com

Here's a paper about a protein that facilitates crosstalk between the nuclear pore and nuclear speckles.

Annals of clinical and translational neurology

Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration

• Cancer
• Imaging
• Methods

Jill Bouchard

Contributing editor, Condensates.com

This article identifies that nuclear speckles may play a key role in this type cancer. I hope it leads to a condensate-based therapeutic!