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Frontiers in molecular biosciences
In vivo Proximity Labeling of Nuclear and Nucleolar Proteins by a Stably Expressed, DNA Damage-Responsive NONO-APEX2 Fusion Protein
Array
Must ReadVIDEO: Bede Portz on An Introduction to Biomolecular Condensates in Disease and to Dewpoint Therapeutics
bioRxiv
Cytoplasmic forces functionally reorganize nuclear condensates in oocytes
Array
Must ReadVIDEO: Emma Lundberg on Mapping the Spatiotemporal Proteome Architecture of Human Cells
BioEssays : news and reviews in molecular, cellular and developmental biology
Chromatin behavior in living cells: Lessons from single-nucleosome imaging and tracking
Proceedings of the National Academy of Sciences of the United States of America
Generation of dynamic three-dimensional genome structure through phase separation of chromatin
Molecular cell
Condensates induced by transcription inhibition localize active chromatin to nucleoli
Development, growth & differentiation
Component Analysis of Nucleolar Protein Compartments using Xenopus laevis oocytes
bioRxiv
CHIP ubiquitin ligase is involved in the nucleolar stress management
Emerging Topics in Biomolecular Magnetic Resonance: Richard Kriwacki & Christian Griesinger
Thursday, May 19, 2022
Biology open
Promyelocytic leukemia nuclear body-like structures can assemble in mouse oocytes
FASEB Science Research Conference–The Nuclear Bodies Conference: Hubs of Genomic Activity (2022)
Sunday, July 24, 2022 | Western Shore, MA, Canada
Array
Must ReadVIDEO: Karla Neugebauer on Nascent RNA and Biomolecular Condensation in the Cell Nucleus
Nature cell biology
Cell cycle-specific phase separation regulated by protein charge blockiness
Sidharth Sirdeshmukh
Research Associate, Dewpoint
The general idea is that the patterns of charge differences in proteins will influence the kinetics of their phase separation-guided reassembly. Charge blocks are stretches of amino acids with similar charge, and ‘blockiness’ is the alternation of blocks of opposite charge (+/-). If we make these blocks shorter, then droplet formation is impeded, and droplets that previously existed dissolve/ diffuse. Hyperphosphorylation during mitosis, lengthens the blocks, which encourages droplet formation. Tuning the lengths, the total number, sequence, and likely stochasticity of these kinds of blocks will likely result in favorable LLPS dynamics/ arrangements for diseases with known condensate MOA.
Cellular and molecular life sciences : CMLS
Nucleolin: a cell portal for viruses, bacteria, and toxins
Molecular cell
Tuning levels of low-complexity domain interactions to modulate endogenous oncogenic transcription
Current opinion in genetics & development
Oncogenic fusion proteins and their role in three-dimensional chromatin structure, phase separation, and cancer
Seminars in cell & developmental biology
Nucleolar stress: From development to cancer
The EMBO journal