Paraspeckles

bioRxiv

Role of spatial patterning of N-protein interactions in SARS-CoV-2 genome packaging

• COVID-19
• Infectious Disease
• Methods
• Phase Separation
• Physics of Condensates

Jill Bouchard

Contributing editor, Condensates.com

The authors looked into whether the arrangement of LLPS-promoting and solubilizing sequences in the genome lead to single genome packaging. Read on to learn about generalizable principles of virus compaction.

RNA biology

Identification and initial characterization of POLIII-driven transcripts by msRNA-sequencing

• Methods

Jill Bouchard

Contributing editor, Condensates.com

It looks like this msRNAseq method may be helpful in identifying medium-sized RNA in other condensates too.

Journal of Parkinson's disease

NEAT1 on the Field of Parkinson’s Disease: Offense, Defense, or a Player on the Bench?

• Neurodegeneration
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

This review also covers NEAT1's role as a scaffold for paraspeckles.

The EMBO journal

Clustering and reverse transcription of HIV-1 genomes in nuclear niches of macrophages

• Imaging
• Infectious Disease
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

It looks like the condensates in infected macrophages get all messed up. Check out this paper to find out more.

Stem cell reports

Chromatin-Associated Membraneless Organelles in Regulation of Cellular Differentiation

• Phase Separation
• Physics of Condensates
• Transcription

bioRxiv

Paraspeckles are constructed as block copolymer micelles through microphase separation

• Physics of Condensates

bioRxiv

Triblock copolymer micelle model of spherical paraspeckles

• Methods
• Physics of Condensates
• Transcription

Non-coding RNA

NEAT1 and Paraspeckles in Cancer Development and Chemoresistance

• Cancer

Molecular reproduction and development

SOX9 is colocalized with paraspeckle protein NONO in cultured murine sertoli cells and features structural characteristics of intrinsically disordered proteins

• Imaging
• Metabolism
• Phase Separation
• Physics of Condensates
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

Here's a nice biophysical analysis of the phase separation of a couple proteins involved in transcription.

International journal of molecular sciences

The Emerging Role of the RNA-Binding Protein SFPQ in Neuronal Function and Neurodegeneration

• Metabolism
• Neurodegeneration
• Phase Separation
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

This review also mentions how phase separation is involved.

Cellular and molecular life sciences : CMLS

NEAT1 polyA-modulating antisense oligonucleotides reveal opposing functions for both long non-coding RNA isoforms in neuroblastoma

• Cancer

Jill Bouchard

Contributing editor, Condensates.com

Here's paper 2/2 this week about the complicated relationship between lncRNA NEAT1 transcripts, paraspeckles, and cancer.

Science advances

Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1

• Cancer

Jill Bouchard

Contributing editor, Condensates.com

Here's paper 1/2 this week about the complicated relationship between lncRNA NEAT1 transcripts, paraspeckles, and cancer.

Essays in biochemistry

Organization and function of paraspeckles

• Cancer
• Phase Separation

Jill Bouchard

Contributing editor, Condensates.com

Here's a nice review breaking down how several proteins make up the core-shell architecture of paraspeckles.

Brain : a journal of neurology

Paraspeckle components NONO and PSPC1 are not mislocalized from motor neuron nuclei in sporadic ALS

• Neurodegeneration

Jill Bouchard

Contributing editor, Condensates.com

Here's some interesting work on how the mislocalization of RNA-binding proteins affects the core-shell structure of paraspeckles.

Neurochemistry international

Pathophysiological Implications of RNP Granules in Frontotemporal Dementia and ALS

• Neurodegeneration
• Physics of Condensates

Jill Bouchard

Contributing editor, Condensates.com

Here's a nice review on the biophysical properties of stress granules and paraspeckles in the hopes of finding a condensate-based therapy for neuodegenerative disorders.

Must Read

Trends in biochemical sciences

Biomolecular Condensates in the Nucleus

• Phase Separation
• Physics of Condensates
• Transcription

Jill Bouchard

Contributing editor, Condensates.com

Need an update on what's new with nuclear condensates? This will be a good place to start!

Molecular cell

Stress Induces Dynamic, Cytotoxicity-Antagonizing TDP-43 Nuclear Bodies via Paraspeckle LncRNA NEAT1-Mediated Liquid-Liquid Phase Separation

• Imaging
• Neurodegeneration
• Phase Separation
• Physics of Condensates

Non-coding RNA

LncRNA NEAT1 in Paraspeckles: A Structural Scaffold for Cellular DNA Damage Response Systems?

• Cancer
• Neurodegeneration
• Phase Separation

Jill Bouchard

Contributing editor, Condensates.com

This review discusses evidence that phase separation plays a role in the assembly of paraspeckles.

Cancer science

Nuclear Microenvironment in Cancer: Control Through Liquid-Liquid Phase Separation

• Cancer
• Methods
• Neurodegeneration
• Phase Separation
• Physics of Condensates

Emerging topics in life sciences

Granule Regulation by Phase Separation During Drosophila Oogenesis

• Phase Separation
• Physics of Condensates