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bioRxiv
The emergence of phase separation as an organizing principle in bacteria
Scientist/ Senior Scientist for Cell Applications & Assay Development (m/f/d)
Dewpoint Therapeutics | Dresden, Germany
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P-bodies are sites of rapid RNA decay during the neural crest epithelial—mesenchymal transition
Jill Bouchard
Contributing editor, Condensates.com
Here's one for the commonly debated question about the role of P bodies: these authors show they're sites for RNA decay.
Journal of visualized experiments : JoVE
Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
Jill Bouchard
Contributing editor, Condensates.com
This could be a really helpful method to monitor condensates.
Screening / Automation Research Associate, Drug Discovery (m/f/d)
Dewpoint Therapeutics | Dresden, Germany
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Dewpoint Therapeutics | Boston, MA, USA
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Dewpoint Therapeutics | Boston, MA, USA
ApplySenior Scientist – HIV Virologist
Dewpoint Therapeutics | Boston, MA, USA
ApplyGroup Leader – Target ID/Interactome Mapping
Dewpoint Therapeutics | Boston, MA USA
ApplyBioEssays : news and reviews in molecular, cellular and developmental biology
Speculating on the Roles of Nuclear Speckles: How RNA-Protein Nuclear Assemblies Affect Gene Expression
Methods in enzymology
A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells
Jill Bouchard
Contributing editor, Condensates.com
Here are some methods that might be useful to track RNA in condensates!
Nature Reviews, Microbiology
Formation and function of bacterial organelles
Jill Bouchard
Contributing editor, Condensates.com
The authors cover both membrane-bound and membrane-less organelles...should be a good read.
The Journal of biological chemistry
Phase separation drives decision making in cell division
Kitchen Table Talk
Christine Mayr on Condensate Networks
bioRxiv
Apolipoprotein E isoform-specific phase transitions in the retinal pigment epithelium drive disease phenotypes in age-related macular degeneration
Jill Bouchard
Contributing editor, Condensates.com
Interesting-- the redox-related phase separation of a cholesterol transporter may lead to the deposits causing macular degeneration. AND(!) therapeutically restoring function just happens to interfere with condensate formation.
Molecular neurodegeneration
FRET-based Tau seeding assay does not represent prion-like templated assembly of Tau filaments
Jill Bouchard
Contributing editor, Condensates.com
This study shows an important consideration for condensate studies: does the tag affect the solubility of the protein of interest?
Proceedings of the National Academy of Sciences of the United States of America
Clusters of bacterial RNA polymerase are biomolecular condensates that assemble through liquid-liquid phase separation
European biophysics journal : EBJ
Fast and synchronized fluctuations of cortical actin negatively correlate with nucleoli liquid-liquid phase separation in T cells
Biochimica et biophysica acta Molecular cell research
Initiation of stress granule assembly by rapid clustering of IGF2BP proteins upon osmotic shock
Jill Bouchard
Contributing editor, Condensates.com
And that clustering is part of a multi-step phase separation process.