bioRxiv
Suppression of liquid-liquid phase separation by 1,6-hexanediol partially compromises the 3D genome organization in living cells
Jill Bouchard
Contributing editor, Condensates.com
This study suggests LLPS fine-tunes 3D genome organization.
Most Recent
3 funded PhD positions on the Role of phase separation mechanisms in higher-order chromatin organization during development
Université de Montpellier | Montpellier, France
ApplybioRxiv
Cingulin unfolds ZO-1 and organizes myosin-2B and gamma-actin to mechanoregulate apical and tight junction membranes
BioRxiv
Chromatin folding variability across single-cells results from state degeneracy in phase-separation
Nature methods
Pooled CRISPR Screens With Imaging on Microraft Arrays Reveals Stress Granule-Regulatory Factors
Jill Bouchard
Contributing editor, Condensates.com
CRISPR + machine learning + stress granules = really cool stuff!
Cells
Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome
Jill Bouchard
Contributing editor, Condensates.com
Who knew that lipids made it into nuclear condensates?!
Kitchen Table Talk
Amy Gladfelter on RNA-Driven Phase Separation
Scientific Reports
Super-resolution in Situ Analysis of Active Ribosomal DNA Chromatin Organization in the Nucleolus
Jill Bouchard
Contributing editor, Condensates.com
The architecture of nucleoli is fascinating, and we'll get so many more insights into how they work as more awesome methods like this come out.
Kitchen Table Talk
Liam Holt on Crowding and Compression
Brain : a journal of neurology
In Vivo Stress Granule Misprocessing Evidenced in a FUS Knock-In ALS Mouse Model
BMC biology
Nucleus Size and DNA Accessibility Are Linked to the Regulation of Paraspeckle Formation in Cellular Differentiation
Jill Bouchard
Contributing editor, Condensates.com
It looks like nuclear lncRNA condensates (including paraspeckles) might serve important roles in cellular regulation and may be targeted therapeutically.
bioRxiv
Functional partitioning of a liquid-like organelle during assembly of axonemal dyneins
Jill Bouchard
Contributing editor, Condensates.com
This work takes a deeper look at a newer condensate called DynAPs.
Science Advances
Nuclear Actin Regulates Inducible Transcription by Enhancing RNA Polymerase II Clustering
Jill Bouchard
Contributing editor, Condensates.com
Here's another way phase separation enables switch-like reactions to external cues.
Kitchen Table Talk
Jeanne Stachowiak on Disordered Proteins and Membrane Traffic
Molecular biology of the cell
Reorganization of Budding Yeast Cytoplasm Upon Energy Depletion
Jill Bouchard
Contributing editor, Condensates.com
Here's an interesting study on how condensates are involved in yeast survival skills.
Kitchen Table Talk
Tatjana Trcek on Germ Granule mRNA Self-Assembly
bioRxiv
Stress induces cytoprotective TDP-43 nuclear bodies through lncRNA NEAT1-promoted phase separation
Methods and applications in fluorescence
High Resolution Mapping of Subcellular Refractive Index by Fluorescence Lifetime Imaging: A Next Frontier in Quantitative Cell Science?
Jill Bouchard
Contributing editor, Condensates.com
Do you plan on using FLIM to study condensates? If so, this article could be particularly useful.
The Journal of biological chemistry
Zinc Promotes Liquid-Liquid Phase Separation of Tau Protein
bioRxiv
Single-molecule imaging reveals translation of mRNAs localized to stress granules
Jill Bouchard
Contributing editor, Condensates.com
Interesting, another data point in the quest to understand what stress granules actually do.