bioRxiv
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BioRxiv
Genome-wide maps of nucleolus interactions reveal distinct layers of repressive chromatin domains
Genome research
Pooled protein tagging, cellular imaging, and in situ sequencing for monitoring drug action in real time
Methods in molecular biology (Clifton, NJ)
Monitoring Acetylation of the RNA Helicase DDX3X, a Protein Critical for Formation of Stress Granules
Jill Bouchard
Contributing editor, Condensates.com
These authors had a pretty stellar Nat Chem Biol paper showing how acetylation regulates phase separation last year. Now they're sharing their protocol--check it out!
Nanoscale
Rapid prediction of drug inhibition under heat stress: single-photon imaging combined with a convolutional neural network
Jill Bouchard
Contributing editor, Condensates.com
This could be a really useful method for anyone wanting to screen drugs that may modulate stress granules.
bioRxiv
Biomolecular Condensation Drives Leukemia Caused by NUP98-Fusion Proteins
bioRxiv
Design of intrinsically disordered proteins that undergo phase transitions with lower critical solution temperatures
Nature communications
Polyelectrolyte interactions enable rapid association and dissociation in high-affinity disordered protein complexes
Jill Bouchard
Contributing editor, Condensates.com
Here's a note to anyone studying condensate-loving IDPs: when highly charged species are involved, concentration can drastically change the kinetics of interactions.
Biophysical journal
The Emergence of Phase Separation as an Organizing Principle in Bacteria
Molecular cell
A Selective Autophagy Pathway for Phase-Separated Endocytic Protein Deposits
Critical reviews in biochemistry and molecular biology
Human de novo purine biosynthesis
Jill Bouchard
Contributing editor, Condensates.com
Here's a review discussing recent evidence showing that the purinosomes are condensates.
bioRxiv
SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNPs from Cajal bodies to nuclear speckles for spliceosome assembly
Jill Bouchard
Contributing editor, Condensates.com
This paper implicates nuclear speckles and cajal bodies in the cellular dysfunctions that may lead to deaf-blindness.
bioRxiv
Synthetic protein condensates that recruit and release protein activity in living cells
Journal of chemical information and modeling
Different Force Fields Give Rise to Different Amyloid Aggregation Pathways in Molecular Dynamics Simulations
Jill Bouchard
Contributing editor, Condensates.com
If you're thinking about using molecular dynamics to investigate aggregation and amyloid formation from condensates, this paper provides some nice insights to consider beforehand.
The Journal of biological chemistry
Hyperosmotic phase separation: Condensates beyond inclusions, granules and organelles
Nature communications
Client proximity enhancement inside cellular membrane-less compartments governed by client-compartment interactions
Frontiers in plant science
A Comprehensive Toolkit for Quick and Easy Visualization of Marker Proteins, Protein-Protein Interactions and Cell Morphology in Marchantia polymorpha
Jill Bouchard
Contributing editor, Condensates.com
Here's a new toolkit for those of you working on condensates in plants. The authors showed the usefulness for P bodies and peroxisomes, and I bet the tools will be useful for studying other condensates as well.
bioRxiv
Mechanistic inferences from analysis of measurements of protein phase transitions in live cells
Science Advances
Small-molecule sequestration of amyloid-β as a drug discovery strategy for Alzheimer’s disease
Proceedings of the National Academy of Sciences of the United States of America