Angewandte Chemie (International ed in English)
Most Recent
bioRxiv
Multivalent interactions between eIF4G1, Pub1 and Pab1 drive the formation of protein condensates
Bio-protocol
Studying Protein Aggregation in the Context of Liquid-liquid Phase Separation Using Fluorescence and Atomic Force Microscopy, Fluorescence and Turbidity Assays, and FRAP
Emerging topics in life sciences
Membraneless organelles: phasing out of equilibrium
bioRxiv
Glycine Rich Segments Adopt Polyproline II Helices Which May Contribute to Biomolecular Condensate Formation
Angewandte Chemie (International ed in English)
Zooming into the Interaction of α-synuclein with Calcium Ions through Exclusively Heteronuclear NMR Experiments
Jill Bouchard
Contributing editor, Condensates.com
Ions play a significant role in the phase behavior of many proteins found in condensates. These methods may help investigate those interactions.
Proceedings of the National Academy of Sciences of the United States of America
Differences in the free energies between the excited states of A β 40 and A β 42 monomers encode their aggregation propensities
Jill Bouchard
Contributing editor, Condensates.com
Using the population of excited states to empirically calculate the aggregation propensity could be a useful approach for predicting whether certain proteins will aggregate in condensates.
International journal of molecular sciences
Development of Novel High-Resolution Size-Guided Turbidimetry-Enabled Particle Purification Liquid Chromatography (PPLC): Extracellular Vesicles and Membraneless Condensates in Focus
Journal of visualized experiments : JoVE
Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
Jill Bouchard
Contributing editor, Condensates.com
This could be a really helpful method to monitor condensates.
Data Scientist, Drug Discovery (m/f/d)
Dewpoint Therapeutics | Dresden, Germany
ApplyGroup Leader – Target ID/Interactome Mapping
Dewpoint Therapeutics | Boston, MA USA
ApplyNature structural & molecular biology
Decoding mRNA translatability and stability from the 5′ UTR
Jill Bouchard
Contributing editor, Condensates.com
Understanding how RNA, particularly untranslated regions, play a role in condendsates is gaining more attention. This method and others like it may be important in learning all there is to know.
Cellular and molecular life sciences : CMLS
Understanding COVID-19 via comparative analysis of dark proteomes of SARS-CoV-2, human SARS and bat SARS-like coronaviruses
Jill Bouchard
Contributing editor, Condensates.com
Check out this article to find out which IDPRs in SARS-CoV-2 are functionally important. I wonder how many of them are also involved in condensates?
International journal of pharmaceutics
First-order nucleation and subsequent growth promote liquid-liquid phase separation of a model IgG1 mAb
Methods in enzymology
A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells
Jill Bouchard
Contributing editor, Condensates.com
Here are some methods that might be useful to track RNA in condensates!
International journal of molecular sciences
Drug Discovery Targeting the Disorder-To-Order Transition Regions through the Conformational Diversity Mimicking and Statistical Analysis
Jill Bouchard
Contributing editor, Condensates.com
Understanding the rules for targeting IDPs will likely also help us drug condensates.
International journal of molecular sciences
Exploring IDP-Ligand Interactions: tau K18 as A Test Case
Jill Bouchard
Contributing editor, Condensates.com
Having a hard time experimentally characterizing interactions between a condensate-loving IDP and possible drug candidates? This paper may give insights into how to successfully probe the interactions.
Nature Reviews, Microbiology
Formation and function of bacterial organelles
Jill Bouchard
Contributing editor, Condensates.com
The authors cover both membrane-bound and membrane-less organelles...should be a good read.
Developmental cell
Phase Separation in Membrane Biology: The Interplay between Membrane-Bound Organelles and Membraneless Condensates
Developmental cell